Anti-RAD50 Polyclonal Antibody (HV313014)

Name: Anti-RAD50 Polyclonal Antibody
Brand: abinScience
SKU: HV313014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HV313014

설명

Anti-RAD50 Polyclonal Antibody (HV313014) is a rabbit polyclonal antibody detecting RAD50 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RAD50 (Thr437-Val792).

타겟

RAD50, hRAD50, DNA repair protein RAD50

정제

Purified by antigen affinity column.

등록번호

Q92878

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA repair protein RAD50 is a ~153 kDa protein. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11.

1. Lee, JH. et al. (2004) Science (New York, N.Y.) 304, 93-6. PMID: 15064416

2. Gatei, M. et al. (2011) The Journal of biological chemistry 286, 31542-56. PMID: 21757780

3. Anand, R. et al. (2016) Molecular cell 64, 940-950. PMID: 27889449

4. Park, YB. et al. (2017) Nature structural & molecular biology 24, 248-257. PMID: 28134932

5. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292

6. Carney, JP. et al. (1998) Cell 93, 477-86. PMID: 9590181

7. Paull, TT. et al. (1998) Molecular cell 1, 969-79. PMID: 9651580

8. Trujillo, KM. et al. (1998) The Journal of biological chemistry 273, 21447-50. PMID: 9705271

9. de Jager, M. et al. (2001) Molecular cell 8, 1129-35. PMID: 11741547

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-RAD50 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: MOLT-4 cell lysate
Lane 4: PC-12 cell lysate

참고 문헌

데이터시트

Anti-RAD50 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-RAD50 Polyclonal Antibody (HV313014)

개요

이미지

참고 문헌

데이터시트

SDS

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