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Anti-REBOV Nucleoprotein Polyclonal Antibody (VK518014)

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개요
카탈로그 번호VK518014
설명
Anti-REBOV Nucleoprotein Polyclonal Antibody (VK518014) is a rabbit polyclonal antibody detecting nucleoprotein in ELISA, IHC, WB. Suitable for Sudan ebolavirus, Reston ebolavirus, and Zaire ebolavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Sudan ebolavirus (SEBOV) (Sudan Ebola virus), Reston ebolavirus (REBOV) (Reston Ebola virus), Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant REBOV Nucleoprotein (Val36-Asn416).
타겟 nucleoprotein, NP
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q5XX08, Q9QP77, Q8JPY1, Q91DE1, O72142, P18272, Q9QCE9
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Nucleoprotein is a ~81 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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