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Anti-RHO Polyclonal Antibody (HY021014)

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개요
카탈로그 번호HY021014
설명
Anti-RHO Polyclonal Antibody (HY021014) is a rabbit polyclonal antibody detecting Rhodopsin; Opsin-2; RHO; OPN2 in ELISA, IHC, WB. Suitable for Human, Dog, Pig, Cat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant human RHO (Asn2-Gln36&Thr97-Cys110&Gly174-Ser202).
타겟 Rhodopsin; Opsin-2; RHO; OPN2
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P08100, P32308, O18766, Q95KU1, P15409, P02700, P51489, P02699, P29403, P28683, P49912
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071
2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847
3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452
4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617
5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343
6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165
7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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