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Anti-Rice CENH3 Polyclonal Antibody (PR109024)

Anti-Rice CENH3 Polyclonal Antibody
Anti-Rice CENH3 Polyclonal Antibody
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개요
카탈로그 번호PR109024
설명
Anti-Rice CENH3 Polyclonal Antibody (PR109024) is a rabbit polyclonal antibody detecting Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Oryza sativa (Rice)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant rice CENH3 (Gly71-Trp164).
타겟 Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q6T367
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Histone H3-like centromeric protein CENH3 is a ~18 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (Probable).

1. Nagaki, K. et al. (2004) Nature genetics 36, 138-45. PMID: 14716315
2. Yan, H. et al. (2006) The Plant cell 18, 2123-33. PMID: 16877494
참고사항 For research use only
이미지
  • Anti-Rice CENH3 Polyclonal Antibody

    Western blot

    Lane 1: HCT116 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Raji cell lysate
    Lane 6: Hela cell lysate
    Lane 7: A375 cell lysate
    Lane 8: A431 cell lysate
    Lane 9: K562 cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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