Anti-RPA1 Polyclonal Antibody (HA686014)

Name: Anti-RPA1 Polyclonal Antibody
Brand: abinScience
SKU: HA686014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HA686014

설명

Anti-RPA1 Polyclonal Antibody (HA686014) is a rabbit polyclonal antibody detecting N-terminally processed; RPA1; REPA1; RPA70 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant human RPA1 (Ile187-Met616).

타겟

Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P27694, Q8VEE4

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Replication protein A 70 kDa DNA-binding subunit (RPA1) is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717

3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720

4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682

5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808

6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923

7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716

8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279

9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650

10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-RPA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate

참고 문헌

데이터시트

Anti-RPA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-RPA1 Polyclonal Antibody (HA686014)

개요

이미지

참고 문헌

데이터시트

SDS