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| 카탈로그 번호 | HB147014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-S100A11 Polyclonal Antibody (HB147014) is a rabbit polyclonal antibody detecting S100A11 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human S100A11 (Met1-Thr105). | ||||||||
| 타겟 | MLN70, Calgizzarin, S100C, MLN 70, Protein S100-C, S100A11, S100 calcium-binding protein A11, Protein S100-A11, Metastatic lymph node gene 70 protein | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P31949 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Protein S100-A11 (S100A11) is a ~11 kDa protein. Facilitates the differentiation and the cornification of keratinocytes. | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting S100A11 (HB147014) at a concentration of 27 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of S100A11 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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