Anti-SARS1 Polyclonal Antibody (HA659014)

Name: Anti-SARS1 Polyclonal Antibody
Brand: abinScience
SKU: HA659014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HA659014

설명

Anti-SARS1 Polyclonal Antibody (HA659014) is a rabbit polyclonal antibody detecting cytoplasmic; 6.1.1.11; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase; SARS1; SARS; SERS in ELISA, IHC, WB. Suitable for Human, Bovine, Rabbit, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant human SARS1 (Ser142-Lys472).

타겟

Serine--tRNA ligase, cytoplasmic; 6.1.1.11; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase; SARS1; SARS; SERS

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P49591, Q9GMB8, P13642, P26638, Q6P799, Q6DRC0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Serine--tRNA ligase, cytoplasmic (SARS1) is a ~58 kDa protein. Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.

1. Xu, X. et al. (2012) Nature communications 3, 681. PMID: 22353712

2. Xu, X. et al. (2013) Structure (London, England : 1993) 21, 2078-86. PMID: 24095058

3. Wang, C. et al. (2015) Nucleic acids research 43, 10534-45. PMID: 26433229

4. Musante, L. et al. (2017) Human mutation 38, 621-636. PMID: 28236339

5. Ravel, JM. et al. (2021) Human mutation 42, 1576-1583. PMID: 34570399

6. Verdura, E. et al. (2022) Journal of medical genetics 59, 1227-1233. PMID: 36041817

7. Vincent, C. et al. (1997) European journal of biochemistry 250, 77-84. PMID: 9431993

8. Shi, Y. et al. (2014) eLife 3, e02349. PMID: 24940000

9. Herzog, W. et al. (2009) Circulation research 104, 1260-6. PMID: 19423847

10. Fukui, H. et al. (2009) Circulation research 104, 1253-9. PMID: 19423848

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-SARS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SARS1 Polyclonal Antibody (HA659014)

개요

이미지

참고 문헌

데이터시트

SDS