Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014)

Name: Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody
Brand: abinScience
SKU: VK022014
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

VK022014

설명

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014) is a rabbit polyclonal antibody detecting Capsid protein in WB, IHC, ELISA. Suitable for O'nyong-nyong virus, Sagiyama virus, Chikungunya virus, Ross river virus, and Middelburg virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Semliki Forest virus (SFV), O'nyong'nyong virus (ONNV), Chikungunya virus (CHIKV), Ross River virus (RRV), Getah virus (GETV), Mayaro virus (MAYV)

응용 분야

WB,IHC,ELISA

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Semliki forest virus/SFV Capsid (Cys119-Trp267).

타겟

Structural polyprotein, p130, Capsid protein, EC:3.4.21.90, Coat protein, C

내독소 수준

Please contact with the lab for this information.

순도

>90% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

P03315, P0DJZ6

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Structural polyprotein (Capsid) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Roussel, A. et al. (2006) Structure (London, England : 1993) 14, 75-86. PMID: 16407067

2. Melancon, P. et al. (1987) Journal of virology 61, 1301-9. PMID: 3553612

3. Skoging, U. et al. (1998) Journal of molecular biology 279, 865-72. PMID: 9642067

4. Ulmanen, I. et al. (1979) Virology 99, 265-76. PMID: 516447

5. Vonderheit, A. et al. (2005) PLoS biology 3, e233. PMID: 15954801

6. Singh, I. et al. (1992) Journal of virology 66, 7049-58. PMID: 1433506

참고사항

For research use only.

이미지

Western blot

Lane 1: recombinant Semliki forest virus/SFV Capsid (Catalog No: VK022012)

참고 문헌

데이터시트

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014)

개요

이미지

참고 문헌

데이터시트

SDS