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| 카탈로그 번호 | HB446014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-SF3A1 Polyclonal Antibody (HB446014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
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| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant human SF3A1 (Ser690-Lys793). | ||||||||
| 타겟 | Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114 | ||||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | A2VDN6, Q15459, Q8K4Z5 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Splicing factor 3A subunit 1 (SF3A1) is a ~88 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes. | ||||||||
| 참고사항 | For research use only |
Western blot analysis was performed using anti-SF3A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Lncap cell lysate
Lane 5: Hela cell lysate
Lane 6: HepG2 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse lung lysate
Lane 10: Mouse testis lysate
Lane11: Rat brain lysate
Lane12: Rat lung lysate
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