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Anti-SHPRH Polyclonal Antibody (HB530014)

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개요
카탈로그 번호HB530014
설명
Anti-SHPRH Polyclonal Antibody (HB530014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase SHPRH; 2.3.2.27; 3.6.4.-; RING-type E3 ubiquitin transferase SHPRH; SNF2 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant human SHPRH (Glu1381-Glu1683).
타겟 E3 ubiquitin-protein ligase SHPRH; 2.3.2.27; 3.6.4.-; RING-type E3 ubiquitin transferase SHPRH; SNF2, histone-linker, PHD and RING finger domain-containing helicase; SHPRH; KIAA2023
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q149N8, Q7TPQ3
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

E3 ubiquitin-protein ligase SHPRH is a ~193 kDa protein. E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.

참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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