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| 카탈로그 번호 | VK002064 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Sindbis virus(SINV) Spike glycoprotein E2 Polyclonal Antibody (VK002064) is a rabbit polyclonal antibody detecting glycoprotein E2 in WB, IHC, ELISA.
Highlights
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| 응용 분야 | WB, IHC, ELISA | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant SINV glycoprotein E2 protein (Ser329-Thr695). | ||||||||
| 타겟 | glycoprotein E2 | ||||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P03316 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. | ||||||||
| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Structural polyprotein (glycoprotein E1) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725 2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066 3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018 4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660 5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569 6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274 7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418 8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418 9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-SINV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Sindbis virus(SINV) Spike glycoprotein E2, N-His (Catalog No: VK002062)
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