Anti-SIRT1 Polyclonal Antibody (HV692014)

Name: Anti-SIRT1 Polyclonal Antibody
Brand: abinScience
SKU: HV692014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HV692014

설명

Anti-SIRT1 Polyclonal Antibody (HV692014) is a rabbit polyclonal antibody detecting SIRT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SIRT1 (Ile194-Ser747).

타겟

NAD-dependent protein deacylase sirtuin-1, hSIR2, NAD-dependent protein deacetylase sirtuin-1, SIR2-like protein 1, 75SirT1, SIR2L1, Regulatory protein SIR2 homolog 1, SIRT1, hSIRT1

정제

Purified by antigen affinity column.

등록번호

Q96EB6

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) is a ~81 kDa protein. NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

1. Vaziri, H. et al. (2001) Cell 107, 149-59. PMID: 11672523

2. Langley, E. et al. (2002) The EMBO journal 21, 2383-96. PMID: 12006491

3. Brunet, A. et al. (2004) Science (New York, N.Y.) 303, 2011-5. PMID: 14976264

4. Motta, MC. et al. (2004) Cell 116, 551-63. PMID: 14980222

5. van der Horst, A. et al. (2004) The Journal of biological chemistry 279, 28873-9. PMID: 15126506

6. Yeung, F. et al. (2004) The EMBO journal 23, 2369-80. PMID: 15152190

7. Cohen, HY. et al. (2004) Science (New York, N.Y.) 305, 390-2. PMID: 15205477

8. Vaquero, A. et al. (2004) Molecular cell 16, 93-105. PMID: 15469825

9. Yang, Y. et al. (2005) The EMBO journal 24, 1021-32. PMID: 15692560

10. Michishita, E. et al. (2005) Molecular biology of the cell 16, 4623-35. PMID: 16079181

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-SIRT1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SIRT1 Polyclonal Antibody (HV692014)

개요

이미지

참고 문헌

데이터시트

SDS

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