Anti-SNAI1 Polyclonal Antibody (HF898014)

Name: Anti-SNAI1 Polyclonal Antibody
Brand: abinScience
SKU: HF898014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HF898014

설명

Anti-SNAI1 Polyclonal Antibody (HF898014) is a rabbit polyclonal antibody detecting SNAI1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SNAI1 (Met1-Arg264).

타겟

Protein sna, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, SNAH

정제

Purified by antigen affinity column.

등록번호

O95863

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Zinc finger protein SNAI1 is a ~29 kDa protein. Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits.

1. Batlle, E. et al. (2000) Nature cell biology 2, 84-9. PMID: 10655587

2. Yook, JI. et al. (2005) The Journal of biological chemistry 280, 11740-8. PMID: 15647282

3. Lin, Y. et al. (2010) The EMBO journal 29, 1803-16. PMID: 20389281

4. Lin, T. et al. (2010) Oncogene 29, 4896-904. PMID: 20562920

5. Zhang, K. et al. (2012) The EMBO journal 31, 29-43. PMID: 21952048

6. Jin, Y. et al. (2015) Cancer letters 362, 70-82. PMID: 25827072

8. Tortorici, M. et al. (2013) ACS chemical biology 8, 1677-82. PMID: 23721412

9. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638

10. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-SNAI1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SNAI1 Polyclonal Antibody (HF898014)

개요

이미지

참고 문헌

데이터시트

SDS