Anti-SPHK1 Polyclonal Antibody (HV674014)

Name: Anti-SPHK1 Polyclonal Antibody
Brand: abinScience
SKU: HV674014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HV674014

설명

Anti-SPHK1 Polyclonal Antibody (HV674014) is a rabbit polyclonal antibody detecting SPHK1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SPHK1 (Ser148-Leu384).

타겟

SPHK, SK 1, SPK, Acetyltransferase SPHK1, Sphingosine kinase 1, SPHK1, SPK 1, SK1

정제

Purified by antigen affinity column.

등록번호

Q9NYA1

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Sphingosine kinase 1 (SPHK1) is a ~42 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion.

1. Gijsbers, S. et al. (2002) Biochimica et biophysica acta 1580, 1-8. PMID: 11923095

2. Alvarez, SE. et al. (2010) Nature 465, 1084-8. PMID: 20577214

3. Wang, Z. et al. (2013) Structure (London, England : 1993) 21, 798-809. PMID: 23602659

4. Shen, H. et al. (2014) Nature cell biology 16, 652-62. PMID: 24929359

5. Lee, JY. et al. (2018) Nature communications 9, 1479. PMID: 29662056

6. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219

7. Adada, MM. et al. (2013) The Journal of biological chemistry 288, 27667-27679. PMID: 23935096

8. Lima, S. et al. (2017) The Journal of biological chemistry 292, 3074-3088. PMID: 28049734

참고사항

For research use only.

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참고 문헌

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Anti-SPHK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SPHK1 Polyclonal Antibody (HV674014)

개요

이미지

참고 문헌

데이터시트

SDS

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