Anti-SPIN1 Polyclonal Antibody (HV382014)

Name: Anti-SPIN1 Polyclonal Antibody
Brand: abinScience
SKU: HV382014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HV382014

설명

Anti-SPIN1 Polyclonal Antibody (HV382014) is a rabbit polyclonal antibody detecting SPIN1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Chicken, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SPIN1 (Met1-Ser262).

타겟

SPIN, SPIN1, Spindlin-1, Ovarian cancer-related protein, Spindlin1, OCR

정제

Purified by antigen affinity column.

등록번호

Q9Y657

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Spindlin-1 (SPIN1) is a ~29 kDa protein. Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and 'Lys-9' (H3K4me3K9me3) and is involved in piRNA-mediated retrotransposon silencing during spermatogenesis. Plays a key role in the initiation of the PIWIL4-piRNA pathway, a pathway that directs transposon DNA methylation and silencing in the male embryonic germ cells, by promoting recruitment of DNA methylation machinery to transposons: binds young, but not old, LINE1 transposons, which are specifically marked with H3K4me3K9me3, and promotes the recruitment of PIWIL4 and SPOCD1 to transposons, leading to piRNA-directed DNA methylation. Also recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability.

1. Du, Y. et al. (2021) Nature communications 12, 949. PMID: 33574238

2. Wang, JX. et al. (2012) Molecular cancer research : MCR 10, 326-35. PMID: 22258766

3. Bae, N. et al. (2017) The Journal of biological chemistry 292, 20808-20817. PMID: 29061846

4. Su, X. et al. (2014) Genes & development 28, 622-36. PMID: 24589551

5. Wang, W. et al. (2011) EMBO reports 12, 1160-6. PMID: 21960006

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-SPIN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SPIN1 Polyclonal Antibody (HV382014)

개요

이미지

참고 문헌

데이터시트

SDS

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