Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

Name: Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody
Brand: abinScience
SKU: JN051014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

JN051014

설명

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014) is a rabbit polyclonal antibody detecting Sortase A in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Staphylococcus aureus

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Staphylococcus aureus srtA/Sortase A (Gln60-Lys206).

타겟

Sortase A, 3.4.22.70, Surface protein sorting A, srtA, SAOUHSC_02834

정제

Purified by antigen affinity column.

등록번호

Q2FV99

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Sortase A (srtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction.

1. Mazmanian, SK. et al. (1999) Science (New York, N.Y.) 285, 760-3. PMID: 10427003

2. Ton-That, H. et al. (1999) The Journal of biological chemistry 274, 24316-20. PMID: 10446208

3. Ton-That, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 12424-9. PMID: 10535938

4. Ton-That, H. et al. (2002) The Journal of biological chemistry 277, 7447-52. PMID: 11714722

5. Kruger, RG. et al. (2004) Biochemistry 43, 1541-51. PMID: 14769030

6. Marraffini, LA. et al. (2004) The Journal of biological chemistry 279, 37763-70. PMID: 15247224

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

개요

이미지

참고 문헌

데이터시트

SDS

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