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Anti-SVA VP1 Polyclonal Antibody (VK547024)

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개요
카탈로그 번호VK547024
설명
Anti-SVA VP1 Polyclonal Antibody (VK547024) is a rabbit polyclonal antibody detecting SVA VP1 in ELISA, IHC, WB. Suitable for Seneca Valley virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Senecavirus A (SVA)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant SVA VP1 (Ser674-Gln937).
타겟 polyprotein, polyprotein
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Purified by antigen affinity column.
등록번호 Q155Z9
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Genome polyprotein (VP1) is a ~240 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. Together they form an icosahedral capsid composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 325 Angstroms (Probable). VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes.

1. Venkataraman, S. et al. (2008) Structure (London, England : 1993) 16, 1555-61. PMID: 18940610
2. Jayawardena, N. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E10934-E10940. PMID: 30381454
3. Cao, L. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 13087-13092. PMID: 30514821
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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