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Anti-TAF7 Polyclonal Antibody (HB463014)

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개요
카탈로그 번호HB463014
설명
Anti-TAF7 Polyclonal Antibody (HB463014) is a rabbit polyclonal antibody detecting Transcription initiation factor TFIID subunit 7; RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit; TAF(II)55; TAFII-55; TAFII55; TAF7; TAF2F; TAFII55 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant human TAF7 (Met1-Ala176).
타겟 Transcription initiation factor TFIID subunit 7; RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit; TAF(II)55; TAFII-55; TAFII55; TAF7; TAF2F; TAFII55
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q15545, Q2HJG8, Q9R1C0
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Transcription initiation factor TFIID subunit 7 (TAF7) is a ~40 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF7 forms a promoter DNA binding subcomplex of TFIID, together with TAF1 and TAF2. Part of a TFIID complex containing TAF10 (TFIID alpha) and a TFIID complex lacking TAF10 (TFIID beta).

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Wu, SY. et al. (1999) The Journal of biological chemistry 274, 23480-90. PMID: 10438527
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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