Anti-TGM2 Polyclonal Antibody (HB247014)

Name: Anti-TGM2 Polyclonal Antibody
Brand: abinScience
SKU: HB247014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB247014

설명

Anti-TGM2 Polyclonal Antibody (HB247014) is a rabbit polyclonal antibody detecting TGM2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TGM2 (Met1-Ser538).

타겟

Protein-glutamine deamidase TGM2, TGase II, Heart G alpha(h), Erythrocyte transglutaminase, Protein-glutamine gamma-glutamyltransferase 2, TG(C), Transglutaminase C, Protein-glutamine serotonyltransferase TGM2, tTG, tTgase, Transglutaminase-2, Protein-glutamine noradrenalinyltransferase TGM2, TGM2, TG2, TGase C, Protein G alpha(h), Protein-glutamine histaminyltransferase TGM2, Tissue transglutaminase, hhG alpha(h), G(h), hTG2, Transglutaminase H, Protein-glutamine dopaminyltransferase TGM2, TGC, Isopeptidase TGM2, TGase-2, TGase H, Transglutaminase II

정제

Purified by antigen affinity column.

등록번호

P21980

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a ~77 kDa protein. Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds.

1. Kanchan, K. et al. (2013) The Biochemical journal 455, 261-72. PMID: 23941696

2. Jeong, EM. et al. (2020) International journal of molecular sciences 21. PMID: 31991788

3. Nemes, Z. et al. (1997) The Journal of biological chemistry 272, 20577-83. PMID: 9252372

4. Upchurch, HF. et al. (1991) Journal of cellular physiology 149, 375-82. PMID: 1683874

5. Lai, TS. et al. (2017) Amino acids 49, 501-515. PMID: 27270573

6. Hernandez-Fernaud, JR. et al. (2017) Nature communications 8, 14206. PMID: 28198360

7. Melino, G. et al. (1994) Molecular and cellular biology 14, 6584-96. PMID: 7935379

8. Hsieh, YF. et al. (2013) PloS one 8, e81516. PMID: 24349085

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TGM2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HUVEC cell lysate
Lane 2: BxPC-3 cell lysate
Lane 3: A549 cell lysate