Anti-TJP1 Polyclonal Antibody (HC432014)

Name: Anti-TJP1 Polyclonal Antibody
Brand: abinScience
SKU: HC432014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HC432014

설명

Anti-TJP1 Polyclonal Antibody (HC432014) is a rabbit polyclonal antibody detecting TJP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TJP1 (Met1-Val190).

타겟

TJP1, ZO1, Zonula occludens protein 1, Zona occludens protein 1, Tight junction protein ZO-1, Tight junction protein 1

정제

Purified by antigen affinity column.

등록번호

Q07157

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Tight junction protein 1 (TJP1) is a ~195 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

1. Furuse, M. et al. (1994) The Journal of cell biology 127, 1617-26. PMID: 7798316

2. Fanning, AS. et al. (1998) The Journal of biological chemistry 273, 29745-53. PMID: 9792688

3. Huo, L. et al. (2011) The EMBO journal 30, 665-78. PMID: 21240187

4. Kremerskothen, J. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 505-14. PMID: 20930113

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TJP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

참고 문헌

데이터시트

Anti-TJP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-TJP1 Polyclonal Antibody (HC432014)

개요

이미지

참고 문헌

데이터시트

SDS