Anti-Mouse TNF-alpha Polyclonal Antibody (MF879014)

Name: Anti-Mouse TNF-alpha Polyclonal Antibody
Brand: abinScience
SKU: MF879014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MF879014

설명

Anti-TNFa/TNF-alpha Polyclonal Antibody (MF879014) is a rabbit polyclonal antibody detecting TNF-alpha in ELISA, IHC, WB. Suitable for Mouse, Rat, and Peromyscus leucopus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse TNFa/TNF-alpha (Leu80-Leu235).

테스트된 응용 분야

타겟

C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form

내독소 수준

Please contact with the lab for this information.

순도

>90% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

P06804

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. TNFa is the therapeutic target of adalimumab (Humira).

1. Ando, Y. et al. (2015) The Journal of biological chemistry 290, 5881-92. PMID: 25586176

2. Jeong, E. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 12894-12906. PMID: 32741026

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TNF polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-12 cell lysate
Lane 2: RAW264.7 cell lysate