Anti-Human p53 Polyclonal Antibody (HY039014)

Name: Anti-Human p53 Polyclonal Antibody
Brand: abinScience
SKU: HY039014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HY039014

설명

Anti-TP53/p53 Polyclonal Antibody (HY039014) is a rabbit polyclonal antibody detecting TP53 in ELISA, IHC, WB. Suitable for Human, Equus asinus, Macaca fascicularis, Macaca mulatta, and Marmota monax.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TP53/p53 (Asp7-Asp393).

테스트된 응용 분야

타겟

Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53

정제

Purified by antigen affinity column.

등록번호

P04637

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

1. Guo, A. et al. (2000) Nature cell biology 2, 730-6. PMID: 11025664

2. Bergamaschi, D. et al. (2003) Nature genetics 33, 162-7. PMID: 12524540

3. Louria-Hayon, I. et al. (2003) The Journal of biological chemistry 278, 33134-41. PMID: 12810724

4. An, W. et al. (2004) Cell 117, 735-48. PMID: 15186775

5. Ghosh, A. et al. (2004) Molecular and cellular biology 24, 7987-97. PMID: 15340061

6. Zhao, Y. et al. (2007) The Journal of biological chemistry 282, 11969-81. PMID: 17317671

7. Taira, N. et al. (2007) Molecular cell 25, 725-38. PMID: 17349958

8. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538

9. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990

10. Wu, L. et al. (2011) The Journal of biological chemistry 286, 2236-44. PMID: 20959462

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TP53 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HT-29 cell lysate
Lane 2: A431 cell lysate
Lane 3: 293T cell lysate
Lane 4: Raji cell lysate

참고 문헌

데이터시트

Anti-Human p53 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human p53 Polyclonal Antibody (HY039014)

개요

이미지

참고 문헌

데이터시트

SDS

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