Anti-TUBB3 Polyclonal Antibody (HC498014)

Name: Anti-TUBB3 Polyclonal Antibody
Brand: abinScience
SKU: HC498014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC498014

설명

Anti-TUBB3 Polyclonal Antibody (HC498014) is a rabbit polyclonal antibody detecting TUBB3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TUBB3 (Met1-Lys450).

타겟

Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain

정제

Purified by antigen affinity column.

등록번호

Q13509

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Tubulin beta-3 chain (TUBB3) is a ~50 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion.

1. LaFrance, BJ. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 34996871

2. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685

3. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661

4. Tischfield, MA. et al. (2010) Cell 140, 74-87. PMID: 20074521

5. Shao, Q. et al. (2017) The Journal of neuroscience : the official journal of the Society for Neuroscience 37, 5620-5633. PMID: 28483977

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TUBB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat testis lysate

참고 문헌

데이터시트

Anti-TUBB3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-TUBB3 Polyclonal Antibody (HC498014)

개요

이미지

참고 문헌

데이터시트

SDS

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