Anti-UBE2A Polyclonal Antibody (HB413014)

Name: Anti-UBE2A Polyclonal Antibody
Brand: abinScience
SKU: HB413014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB413014

설명

Anti-UBE2A Polyclonal Antibody (HB413014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A in ELISA, IHC, WB. Suitable for Human, Mouse, Rabbit, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant human UBE2A (Met1-Cys152).

타겟

Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P49459, Q9Z255, P63146, P63148, P63149, P63147, Q32P99, P25153, P52478

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Ubiquitin-conjugating enzyme E2 A (UBE2A) is a ~17 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN.

1. Kim, J. et al. (2005) Molecular cell 20, 759-70. PMID: 16337599

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Haddad, DM. et al. (2013) Molecular cell 50, 831-43. PMID: 23685073

4. Hong, JH. et al. (2015) Molecular & cellular proteomics : MCP 14, 674-85. PMID: 25582440

5. Haakonsen, DL. et al. (2024) Nature 626, 874-880. PMID: 38297121

6. Barnsby-Greer, L. et al. (2024) Nature structural & molecular biology 31, 351-363. PMID: 38182926

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-UBE2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: A375 cell lysate
Lane 5: SW620 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: N2A cell lysate
Lane 8: NIH3T3 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate

참고 문헌

데이터시트

Anti-UBE2A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-UBE2A Polyclonal Antibody (HB413014)

개요

이미지

참고 문헌

데이터시트

SDS