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Anti-UBE2H Polyclonal Antibody (HB330014)

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  • 개요

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개요
카탈로그 번호HB330014
설명
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant human UBE2H (Met1-Leu183).
타겟 Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P62256, P62257, Q32LN1
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Ubiquitin-conjugating enzyme E2 H (UBE2H) is a ~20 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

1. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522
2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
3. Kaiser, P. et al. (1994) The Journal of biological chemistry 269, 8797-802. PMID: 8132613
4. Lampert, F. et al. (2018) eLife 7. PMID: 29911972
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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