Anti-VDAC1 Polyclonal Antibody (HB031014)

Name: Anti-VDAC1 Polyclonal Antibody
Brand: abinScience
SKU: HB031014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HB031014

설명

Anti-VDAC1 Polyclonal Antibody (HB031014) is a rabbit polyclonal antibody detecting VDAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human VDAC1 (Met1-Ala283).

타겟

Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, hVDAC1, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC1, VDAC-1, VDAC

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P21796, Q60932, Q9Z2L0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Non-selective voltage-gated ion channel VDAC1 is a ~30 kDa protein. Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol.

1. Stadtmüller, U. et al. (1999) Biological chemistry 380, 1461-6. PMID: 10661876

2. Thinnes, FP. et al. (2001) Pflugers Archiv : European journal of physiology 443 Suppl 1, S111-6. PMID: 11845315

3. Hiller, S. et al. (2008) Science (New York, N.Y.) 321, 1206-10. PMID: 18755977

4. Marginedas-Freixa, I. et al. (2018) Scientific reports 8, 11384. PMID: 30061676

5. Blachly-Dyson, E. et al. (1993) The Journal of biological chemistry 268, 1835-41. PMID: 8420959

6. Dadsena, S. et al. (2019) Nature communications 10, 1832. PMID: 31015432

7. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033

10. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-VDAC1 polyclonal antibody at 1µg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: Mouse brain lysate
Lane 4: Rat brain lysate
Lane 5: Mouse heart lysate
Lane 6: Rat heart lysate

참고 문헌

데이터시트

Anti-VDAC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-VDAC1 Polyclonal Antibody (HB031014)

개요

이미지

참고 문헌

데이터시트

SDS

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