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Anti-VSV RdRp/Protein L Polyclonal Antibody (VK422034)

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개요
카탈로그 번호VK422034
설명
Anti-VSIV RdRp/Protein L Polyclonal Antibody (VK422034) is a rabbit polyclonal antibody detecting VSIV RdRp/Protein L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Vesicular stomatitis Indiana virus (VSIV)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant VSIV RdRp/Protein L (Glu2-Gly188).
타겟 polymerase, partial, polymerase, partial
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q8B0I0, Q8B0H5, Q8B0H0, Q98776, P03523
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

RNA-directed RNA polymerase L (RdRp/Protein L) is a ~240 kDa protein. Multifunctional enzyme responsible for RNA synthesis (replicase and transcriptase), cap addition, and cap methylation. Also performs the polyadenylation of subgenomic mRNAs by a stuttering mechanism at a slipery stop site present at the end of viral genes. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). L is packaged into virions during assembly and translocates to the 3' leader promoter to initiate transcription after entering the host cells. During transcription and replication of the genome, L does not bind the N-RNA complex directly, but is bridged by its non-catalytic cofactor P, which interacts with L and N oligomers simultaneously.

참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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