Anti-XIAP Polyclonal Antibody (HX165014)

Name: Anti-XIAP Polyclonal Antibody
Brand: abinScience
SKU: HX165014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HX165014

설명

Anti-XIAP Polyclonal Antibody (HX165014) is a rabbit polyclonal antibody detecting XIAP in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human XIAP (Met1-Ser497).

타겟

ILP, IAP-like protein, X-linked inhibitor of apoptosis protein, IAP-3, hILP, X-linked IAP, Baculoviral IAP repeat-containing protein 4, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, E3 ubiquitin-protein ligase XIAP, API3, XIAP, hIAP-3, hIAP3, BIRC4, IAP3

정제

Purified by antigen affinity column.

등록번호

P98170

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E3 ubiquitin-protein ligase XIAP is a ~56 kDa protein. Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS, PTEN and BIRC5/survivin.

1. Chai, J. et al. (2001) Cell 104, 769-80. PMID: 11257230

2. Huang, Y. et al. (2001) Cell 104, 781-90. PMID: 11257231

3. Suzuki, Y. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 8662-7. PMID: 11447297

4. MacFarlane, M. et al. (2002) The Journal of biological chemistry 277, 36611-6. PMID: 12121969

5. Shiozaki, EN. et al. (2003) Molecular cell 11, 519-27. PMID: 12620238

6. Lu, M. et al. (2007) Molecular cell 26, 689-702. PMID: 17560374

7. Wilkinson, JC. et al. (2008) Molecular and cellular biology 28, 237-47. PMID: 17967870

8. Van Themsche, C. et al. (2009) The Journal of biological chemistry 284, 20462-6. PMID: 19473982

9. Brady, GF. et al. (2010) Molecular and cellular biology 30, 1923-36. PMID: 20154138

10. Lewis, EM. et al. (2011) Biochemistry 50, 11084-96. PMID: 22103349

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-XIAP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-XIAP Polyclonal Antibody (HX165014)

개요

이미지

참고 문헌

데이터시트

SDS

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