Anti-YWHAZ Polyclonal Antibody (HX859014)

Name: Anti-YWHAZ Polyclonal Antibody
Brand: abinScience
SKU: HX859014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HX859014

설명

Anti-YWHAZ Polyclonal Antibody (HX859014) is a rabbit polyclonal antibody detecting YWHAZ in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

타겟

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

정제

Purified by antigen affinity column.

등록번호

P63104

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

14-3-3 protein zeta/delta (YWHAZ) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

1. Zheng, W. et al. (2003) Nature structural biology 10, 1054-7. PMID: 14578935

2. Tsuruta, F. et al. (2004) The EMBO journal 23, 1889-99. PMID: 15071501

3. Ganguly, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1222-7. PMID: 15644438

4. Gu, YM. et al. (2006) FEBS letters 580, 305-10. PMID: 16376338

5. Angrand, PO. et al. (2006) Molecular & cellular proteomics : MCP 5, 2211-27. PMID: 16959763

6. Popov, IK. et al. (2019) Frontiers in physiology 10, 388. PMID: 31024343

7. Dubois, T. et al. (1997) The Journal of biological chemistry 272, 28882-8. PMID: 9360956

8. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-YWHAZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: Raji cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse kidney lysate
Lane11: Rat brain lysate
Lane12: Rat kidney lysate

참고 문헌

데이터시트

Anti-YWHAZ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-YWHAZ Polyclonal Antibody (HX859014)

개요

이미지

참고 문헌

데이터시트

SDS