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| 카탈로그 번호 | VK512014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Zaire ebolavirus (ZEBOV) (Zaire Ebola virus) | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351). | ||||||||
| 타겟 | Nucleoprotein, Nucleocapsid protein, NP | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | O72142, P18272 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm. | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-Ebola virus Nucleoprotein polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 70 ug
Lane 2: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 50 ug
Lane 3: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 30 ug
Lane 4: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 10 ug
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