HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818)

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카탈로그 번호	HX206818
설명	HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818) is a stable cell line expressing CD184/CXCR4. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	P61073
응용 분야	FCM
숙주 종	HEK293T
종	Human
타겟	CD184/CXCR4
검출 방법	Colorimetric
샘플 유형	Plasma, Serum
분석 유형	Quantitative
범위	1.56 - 100 ng/mL
민감도	0.25 ng/mL
회수율	80-120%
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
배송	2-8 ℃
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	C-X-C chemokine receptor type 4 (CD184/CXCR4) is a ~39 kDa protein. Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. CXCR4 is coupled to G(i) G alpha proteins and mediates inhibition of adenylate cyclase. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. 1. Brelot, A. et al. (1999) Journal of virology 73, 2576-86. PMID: 10074102 2. Gupta, SK. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 2368-72. PMID: 10452968 3. Cheng, ZJ. et al. (2000) The Journal of biological chemistry 275, 2479-85. PMID: 10644702 4. Brelot, A. et al. (2000) The Journal of biological chemistry 275, 23736-44. PMID: 10825158 5. Veldkamp, CT. et al. (2008) Science signaling 1, ra4. PMID: 18799424 6. Busillo, JM. et al. (2010) The Journal of biological chemistry 285, 7805-17. PMID: 20048153 7. Malik, R. et al. (2010) Molecular biology of the cell 21, 2529-41. PMID: 20505072 8. Cao, Y. et al. (2015) Leukemia 29, 169-76. PMID: 24912431 9. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524 10. Bleul, CC. et al. (1996) Nature 382, 829-33. PMID: 8752280
참고사항	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CXCR4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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