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| 카탈로그 번호 | VK475012 |
|---|---|
| 설명 |
Recombinant ASFV UBC/pI215L Protein, N-His (VK475012) expressed in E. coli, spanning Met1-Thr200. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P27949 |
| 단백질 길이 | Met1-Thr200 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | African swine fever virus (ASFV) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 25.01 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L |
| 배경 | Ubiquitin-conjugating enzyme E2 (UBC/pI215L) is a ~24 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication. May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery. Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling. 1. Freitas, FB. et al. (2018) Scientific reports 8, 3471. PMID: 29472632 2. Barrado-Gil, L. et al. (2020) Frontiers in microbiology 11, 622907. PMID: 33384682 3. Barrado-Gil, L. et al. (2021) Viruses 13. PMID: 34204411 4. Huang, L. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 2754-2769. PMID: 34759016 5. Hingamp, PM. et al. (1995) Journal of virology 69, 1785-93. PMID: 7853518 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant ASFV UBC/pI215L Protein, N-His
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