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| 카탈로그 번호 | HB443012 |
|---|---|
| 설명 |
Recombinant Human ADPRS Protein, N-His (HB443012) expressed in E. coli, spanning Ser19-Ser363. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9NX46 |
| 단백질 길이 | Ser19-Ser363 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 39.81 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3 |
| 배경 | ADP-ribosylhydrolase ARH3 is a ~38 kDa protein. ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos. 1. Kasamatsu, A. et al. (2011) The Journal of biological chemistry 286, 21110-7. PMID: 21498885 2. Pourfarjam, Y. et al. (2018) The Journal of biological chemistry 293, 12350-12359. PMID: 29907568 3. Wang, M. et al. (2018) The Journal of biological chemistry 293, 14470-14480. PMID: 30045870 4. Danhauser, K. et al. (2018) American journal of human genetics 103, 817-825. PMID: 30401461 5. Mashimo, M. et al. (2019) JCI insight 4. PMID: 30830864 6. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521 7. Voorneveld, J. et al. (2021) Chemistry (Weinheim an der Bergstrasse, Germany) 27, 10621-10627. PMID: 33769608 8. Pourfarjam, Y. et al. (2021) The Journal of biological chemistry 296, 100692. PMID: 33894202 9. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811 10. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462 |
| 참고사항 | For research use only |
SDS-PAGE for Recombinant Human ADPRS Protein, N-His
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