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| 카탈로그 번호 | HT110012 |
|---|---|
| 설명 |
Recombinant Human AKAP8 Protein, N-GST (HT110012) expressed in E. coli, spanning Met1-Glu572. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O43823 |
| 단백질 길이 | Met1-Glu572 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 91.06 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | AKAP8, A-kinase anchor protein 8, AKAP95, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95 |
| 배경 | A-kinase anchor protein 8 (AKAP8) is a ~76 kDa protein. Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. 1. Eide, T. et al. (1998) Experimental cell research 238, 305-16. PMID: 9473338 2. Collas, P. et al. (1999) The Journal of cell biology 147, 1167-80. PMID: 10601332 3. Steen, RL. et al. (2000) The Journal of cell biology 149, 531-6. PMID: 10791967 4. Eide, T. et al. (2002) EMBO reports 3, 426-32. PMID: 11964380 5. Arsenijevic, T. et al. (2004) The Biochemical journal 378, 673-9. PMID: 14641107 6. Li, Y. et al. (2006) Genes & development 20, 2566-79. PMID: 16980585 7. Gao, X. et al. (2012) Molecular biology of the cell 23, 503-15. PMID: 22130794 8. Eide, T. et al. (2003) The Journal of biological chemistry 278, 26750-6. PMID: 12740381 9. Jiang, H. et al. (2013) Nature structural & molecular biology 20, 1156-63. PMID: 23995757 10. Kamada, S. et al. (2005) Molecular and cellular biology 25, 9469-77. PMID: 16227597 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human AKAP8 Protein, N-GST
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