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| 카탈로그 번호 | HY127012 |
|---|---|
| 설명 |
Recombinant Human ALPL Protein, N-His (HY127012) expressed in E. coli, spanning Leu18-Ser501. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P05186 |
| 단백질 길이 | Leu18-Ser501 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 55.59 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Alkaline phosphatase liver/bone/kidney isozyme, AP-TNAP, TNSALP, Alkaline phosphatase, tissue-nonspecific isozyme, ALPL |
| 배경 | Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) is a ~57 kDa protein. Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. 1. Di Mauro, S. et al. (2002) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 17, 1383-91. PMID: 12162492 2. Sultana, S. et al. (2013) Molecular genetics and metabolism 109, 282-8. PMID: 23688511 3. Numa-Kinjoh, N. et al. (2015) Molecular genetics and metabolism 115, 180-5. PMID: 25982064 4. Fedde, KN. et al. (1990) American journal of human genetics 47, 767-75. PMID: 2220817 5. Balasubramaniam, S. et al. (2010) Journal of inherited metabolic disease 33 Suppl 3, S25-33. PMID: 20049532 6. Pettengill, M. et al. (2017) PloS one 12, e0175936. PMID: 28448526 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human ALPL Protein, N-His
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