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| 카탈로그 번호 | HA353012 |
|---|---|
| 설명 |
Recombinant Human ATF5 Protein, N-His (HA353012) expressed in E. coli, spanning Pro96-Cys282. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9Y2D1 |
| 단백질 길이 | Pro96-Cys282 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 23.25 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx, ATF5, ATFX |
| 배경 | Cyclic AMP-dependent transcription factor ATF-5 (ATF5) is a ~30 kDa protein. Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. 1. Pati, D. et al. (1999) Molecular and cellular biology 19, 5001-13. PMID: 10373550 2. Liu, W. et al. (2004) Biochemical and biophysical research communications 321, 954-60. PMID: 15358120 3. Yamazaki, T. et al. (2010) Life sciences 87, 294-301. PMID: 20654631 4. Dluzen, D. et al. (2011) The Journal of biological chemistry 286, 7705-13. PMID: 21212266 5. Leong, DT. et al. (2012) Journal of cellular biochemistry 113, 2744-53. PMID: 22442021 6. Liu, DX. et al. (2011) Molecular and cellular biology 31, 3906-16. PMID: 21791614 7. Pascual, M. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 1063-72. PMID: 18332083 8. Gho, JW. et al. (2008) Cancer research 68, 6743-51. PMID: 18701499 9. Liu, X. et al. (2012) The Journal of biological chemistry 287, 19599-609. PMID: 22528486 |
| 참고사항 | For research use only. |
SDS-PAGE for Human ATF5 Recombinant Protein (N-His)
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