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| 카탈로그 번호 | HT394012 |
|---|---|
| 설명 |
Recombinant Human AURKA Protein, N-His (HT394012) expressed in E. coli, spanning Ala129-Ser403. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O14965 |
| 단백질 길이 | Ala129-Ser403 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 33.99 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Aurora kinase A, 2.7.11.1, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, Breast tumor-amplified kinase, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, AURKA, AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6 |
| 배경 | Aurora kinase A (AURKA) is a ~45 kDa protein. Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. 1. Walter, AO. et al. (2000) Oncogene 19, 4906-16. PMID: 11039908 2. Marumoto, T. et al. (2002) Genes to cells : devoted to molecular & cellular mechanisms 7, 1173-82. PMID: 12390251 3. Fancelli, D. et al. (2006) Journal of medicinal chemistry 49, 7247-51. PMID: 17125279 4. Manfredi, MG. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 4106-11. PMID: 17360485 5. Macůrek, L. et al. (2008) Nature 455, 119-23. PMID: 18615013 6. Carvalhal, S. et al. (2015) Molecular biology of the cell 26, 3424-38. PMID: 26246606 7. Marumoto, T. et al. (2003) The Journal of biological chemistry 278, 51786-95. PMID: 14523000 8. Kotak, S. et al. (2016) Journal of cell science 129, 3015-25. PMID: 27335426 9. Hirota, T. et al. (2003) Cell 114, 585-98. PMID: 13678582 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human AURKA Protein, N-His.
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