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| 카탈로그 번호 | HA695012 |
|---|---|
| 설명 |
Recombinant Human BRCC3 Protein, N-His (HA695012) expressed in E. coli, spanning Val12-Thr223. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P46736 |
| 단백질 길이 | Val12-Thr223 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 26.56 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53 |
| 배경 | Lys-63-specific deubiquitinase BRCC36 is a ~36 kDa protein. Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. 1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193 2. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690 3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985 4. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097 5. Dong, Y. et al. (2003) Molecular cell 12, 1087-99. PMID: 14636569 6. Chen, X. et al. (2006) Cancer research 66, 5039-46. PMID: 16707425 7. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341 9. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746 10. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748 |
| 참고사항 | For research use only |
SDS-PAGE for Recombinant Human BRCC3 Protein, N-His
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