Human CTLA-4 Recombinant Protein (C-Fc) (HB651021)

Name: Human CTLA-4 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: HB651021
Price: 298 USD
Availability: InStock

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사양:

50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB651021
설명	Recombinant Human CD152/CTLA4 Protein, C-Fc (HB651021) expressed in Mammalian Cells, spanning Met1-Phe162. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	Mammalian cells
등록번호	P16410
단백질 길이	Met1-Phe162
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
타겟	HA, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	44.13 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	CD152, CTLA-4, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
배경	Cytotoxic T-lymphocyte protein 4 is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. 1. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501 2. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502 3. Teft, WA. et al. (2006) Annual review of immunology 24, 65-97. PMID: 16551244 4. Linsley, PS. et al. (1991) The Journal of experimental medicine 174, 561-9. PMID: 1714933 5. Zheng, Y. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1683-91. PMID: 18641304 6. Ramagopal, UA. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E4223-E4232. PMID: 28484017
참고사항	For research use only

이미지

SDS-PAGE

SDS-PAGE for Recombinant Human CD152/CTLA4 Protein, C-Fc
Bioactivity

Immobilized HW630021 Human CD80/B7-1 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB651021 Human CD152/CTLA4 Recombinant Protein (C-Fc), the EC 50 is 143 ng/mL.
Bioactivity

Immobilized MW630021 Mouse CD80/B7-1 Recombinant Protein (C-His-GPI) at 2 μg/mL(100 μL/well) can bind HB651021 Human CD152/CTLA4 Recombinant Protein (C-Fc), the EC 50 is 46.0 ng/mL.