Human CD82 Recombinant Protein (N-His) (HB018012)

Name: Human CD82 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB018012
Price: 211 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB018012
説明	Recombinant Human CD82 Protein, N-His (HB018012) expressed in E. coli, spanning Gly111-Leu228. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	P27701
タンパク質長	Gly111-Leu228
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	15.84 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27, CD82, CD82, KAI1, SAR2, ST6, TSPAN27
背景	CD82 antigen is a ~29 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization. Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC. Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4. 1. Xu, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3273-88. PMID: 19497983 2. Odintsova, E. et al. (2000) Current biology : CB 10, 1009-12. PMID: 10985391 3. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 4. Odintsova, E. et al. (2013) The Journal of biological chemistry 288, 26323-26334. PMID: 23897813 5. Termini, CM. et al. (2014) Molecular biology of the cell 25, 1560-73. PMID: 24623721 6. Mannion, BA. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 2039-47. PMID: 8757325 7. He, B. et al. (2005) The Journal of biological chemistry 280, 3346-54. PMID: 15557282 8. Jee, BK. et al. (2007) Biochemical and biophysical research communications 359, 703-8. PMID: 17560548 9. Bass, R. et al. (2005) The Journal of biological chemistry 280, 14811-8. PMID: 15677461 10. Lee, H. et al. (2023) BMB reports 56, 359-364. PMID: 36945827
注意事項	For research use only.