Anti-Human CD82 Polyclonal Antibody (HB018014)

Name: Anti-Human CD82 Polyclonal Antibody
Brand: abinScience
SKU: HB018014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB018014

Description

Anti-Human CD82 Polyclonal Antibody (HB018014) is a rabbit polyclonal antibody detecting CD82 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD82 (Gly111-Leu228).

Target

CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27, CD82, CD82, KAI1, SAR2, ST6, TSPAN27

Purification

Purified by antigen affinity column.

Accession

P27701

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD82 antigen is a ~29 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization. Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC. Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4.

1. Xu, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3273-88. PMID: 19497983

2. Odintsova, E. et al. (2000) Current biology : CB 10, 1009-12. PMID: 10985391

3. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033

4. Odintsova, E. et al. (2013) The Journal of biological chemistry 288, 26323-26334. PMID: 23897813

5. Termini, CM. et al. (2014) Molecular biology of the cell 25, 1560-73. PMID: 24623721

6. Mannion, BA. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 2039-47. PMID: 8757325

7. He, B. et al. (2005) The Journal of biological chemistry 280, 3346-54. PMID: 15557282

8. Jee, BK. et al. (2007) Biochemical and biophysical research communications 359, 703-8. PMID: 17560548

9. Bass, R. et al. (2005) The Journal of biological chemistry 280, 14811-8. PMID: 15677461

10. Lee, H. et al. (2023) BMB reports 56, 359-364. PMID: 36945827

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD82 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: SW480 cell lysate
Lane 3: Mosue brain lysate
Lane 4: Rat brain lysate