Recombinant Human CD82 Protein, C-His (HB018011) expressed in Mammalian Cells, spanning Gly111-Leu228. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
P27701
Protein length
Gly111-Leu228
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
16.74 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD82 antigen is a ~29 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization. Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC. Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4.
1. Xu, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3273-88. PMID: 19497983
2. Odintsova, E. et al. (2000) Current biology : CB 10, 1009-12. PMID: 10985391
3. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033
4. Odintsova, E. et al. (2013) The Journal of biological chemistry 288, 26323-26334. PMID: 23897813
5. Termini, CM. et al. (2014) Molecular biology of the cell 25, 1560-73. PMID: 24623721
6. Mannion, BA. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 2039-47. PMID: 8757325
7. He, B. et al. (2005) The Journal of biological chemistry 280, 3346-54. PMID: 15557282
8. Jee, BK. et al. (2007) Biochemical and biophysical research communications 359, 703-8. PMID: 17560548
9. Bass, R. et al. (2005) The Journal of biological chemistry 280, 14811-8. PMID: 15677461
10. Lee, H. et al. (2023) BMB reports 56, 359-364. PMID: 36945827
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD82 Protein, C-His
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