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| 카탈로그 번호 | HX024012 |
|---|---|
| 설명 |
Recombinant Human CENPE Protein, N-His (HX024012) expressed in E. coli, spanning Ala2450-Arg2590. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q02224 |
| 단백질 길이 | Ala2450-Arg2590 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 18.64 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | CENP-E, CENPE, Kinesin-related protein CENPE, Centromere-associated protein E, Centromere protein E, Kinesin-7 |
| 배경 | Centromere-associated protein E (CENPE) is a ~316 kDa protein. Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized. 1. Shrestha, RL. et al. (2013) Current biology : CB 23, 1514-26. PMID: 23891108 2. Bancroft, J. et al. (2015) Journal of cell science 128, 171-84. PMID: 25395579 3. Thrower, DA. et al. (1995) The EMBO journal 14, 918-26. PMID: 7889940 4. Barisic, M. et al. (2015) Science (New York, N.Y.) 348, 799-803. PMID: 25908662 5. Gudimchuk, N. et al. (2013) Nature cell biology 15, 1079-1088. PMID: 23955301 6. Iemura, K. et al. (2015) Nature communications 6, 6447. PMID: 25743205 7. Liu, D. et al. (2007) The Journal of biological chemistry 282, 21415-24. PMID: 17535814 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CENPE Protein, N-His
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