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| 카탈로그 번호 | HW489012 |
|---|---|
| 설명 |
Recombinant Human Clcn1 Protein, N-His (HW489012) expressed in E. coli, spanning Leu587-Gly877. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P35523 |
| 단백질 길이 | Leu587-Gly877 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 34.51 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | CLC1, CLCN1, Chloride channel protein 1, Chloride channel protein, skeletal muscle, ClC-1 |
| 배경 | Chloride channel protein 1 is a ~108 kDa protein. Voltage-gated chloride channel involved in skeletal muscle excitability. Generates most of the plasma membrane chloride conductance in skeletal muscle fibers, stabilizes the resting membrane potential and contributes to the repolarization phase during action potential firing. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Has a significant open probability at muscle resting potential and is further activated upon membrane depolarization. 1. Ryan, A. et al. (2002) The Journal of physiology 545, 345-54. PMID: 12456816 2. Bennetts, B. et al. (2005) The Journal of biological chemistry 280, 32452-8. PMID: 16027167 3. Ulzi, G. et al. (2012) Journal of the neurological sciences 318, 65-71. PMID: 22521272 4. Weinberger, S. et al. (2012) The Journal of physiology 590, 3449-64. PMID: 22641783 5. Portaro, S. et al. (2015) Neuromolecular medicine 17, 285-96. PMID: 26007199 6. Ronstedt, K. et al. (2015) Scientific reports 5, 15382. PMID: 26502825 7. Vindas-Smith, R. et al. (2016) Human mutation 37, 74-83. PMID: 26510092 8. Lorenz, C. et al. (1994) Human molecular genetics 3, 941-6. PMID: 7951242 9. Steinmeyer, K. et al. (1994) The EMBO journal 13, 737-43. PMID: 8112288 10. Pusch, M. et al. (1994) Biophysical journal 66, 149-52. PMID: 8130334 |
| 참고사항 | For research use only |
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