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| 카탈로그 번호 | HB959012 |
|---|---|
| 설명 |
Recombinant Human CYP11B1 Protein, N-GST (HB959012) expressed in E. coli, spanning Gly134-Asn503. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P15538 |
| 단백질 길이 | Gly134-Asn503 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 68.99 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | CYP11B1, Cytochrome P-450c11, Steroid 11-beta-hydroxylase, CYP11B1, Cytochrome P450C11, CYPXIB1, Cytochrome P450 11B1, mitochondrial, S11BH |
| 배경 | Cytochrome P450 11B1, mitochondrial (CYP11B1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. 1. Bureik, M. et al. (2002) Endocrine research 28, 351-5. PMID: 12530636 2. Pascoe, L. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 8327-31. PMID: 1518866 3. Curnow, KM. et al. (1991) Molecular endocrinology (Baltimore, Md.) 5, 1513-22. PMID: 1775135 4. Zöllner, A. et al. (2008) The FEBS journal 275, 799-810. PMID: 18215163 5. Strushkevich, N. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 315-24. PMID: 23322723 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CYP11B1 Protein, N-GST
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