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| 카탈로그 번호 | HD954012 |
|---|---|
| 설명 |
Recombinant Human CYP27A1 Protein, N-His (HD954012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q02318 |
| 단백질 길이 | Arg52-Cys531 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CYP27, CYP27A1, Cytochrome P-450C27/25, Cytochrome P450 27, EC:1.14.15.15, Sterol 26-hydroxylase, mitochondrial, Sterol 27-hydroxylase, Vitamin D(3) 25-hydroxylase |
| 배경 | Sterol 26-hydroxylase, mitochondrial is a ~60 kDa protein. Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism. Plays a role in cholestanol metabolism in the cerebellum. 1. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124 2. Heo, GY. et al. (2011) Journal of lipid research 52, 1117-1127. PMID: 21411718 3. Mast, N. et al. (2017) The Journal of biological chemistry 292, 4913-4924. PMID: 28190002 4. Pikuleva, IA. et al. (1998) The Journal of biological chemistry 273, 18153-60. PMID: 9660774 5. Pikuleva, IA. et al. (2001) Biochemistry 40, 7621-9. PMID: 11412116 6. Cali, JJ. et al. (1991) The Journal of biological chemistry 266, 7774-8. PMID: 1708392 7. Cali, JJ. et al. (1991) The Journal of biological chemistry 266, 7779-83. PMID: 2019602 8. Kim, KS. et al. (1994) Journal of lipid research 35, 1031-9. PMID: 7915755 9. Chen, W. et al. (1997) Journal of lipid research 38, 870-9. PMID: 9186905 |
| 참고사항 | For research use only |
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