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Human CYP4F8 Recombinant Protein (N-His) (HD835012)

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사양:
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HD835012
설명
Recombinant Human CYP4F8 Protein, N-His (HD835012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
발현 시스템E. coli
등록번호P98187
단백질 길이Thr38-Gly520
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CYP4F8, CYPIVF8, Cytochrome P450 4F8, EC:1.14.14.1
배경

Cytochrome P450 4F8 is a ~59 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-1 and omega-2 positions. Hydroxylates (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) predominantly at omega-2 position to form (18R)-hydroxyeicosatetraenoic acid (18R-HETE). Exhibits omega-1 hydroxylase activity toward prostaglandin (PG) H1, PGH2 and PGI2.

1. Bylund, J. et al. (2000) The Journal of biological chemistry 275, 21844-9. PMID: 10791960
2. Stark, K. et al. (2005) Prostaglandins & other lipid mediators 75, 47-64. PMID: 15789615
3. Stark, K. et al. (2005) Archives of biochemistry and biophysics 441, 174-81. PMID: 16112640
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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