中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HC204012 |
|---|---|
| 설명 |
Recombinant Human CYP7A1 Protein, N-His (HC204012) expressed in E. coli, spanning Gly43-Asp397. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P22680 |
| 단백질 길이 | Gly43-Asp397 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 42.84 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 24-hydroxycholesterol 7-alpha-hydroxylase, CYP7, CYP7A1, CYPVII, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, Cytochrome P450 7A1, EC:1.14.14.23, EC:1.14.14.26 |
| 배경 | Cytochrome P450 7A1 is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. 1. Norlin, M. et al. (2000) Journal of lipid research 41, 1629-39. PMID: 11013305 2. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124 3. Li, T. et al. (2010) Journal of lipid research 51, 832-42. PMID: 19965590 4. Shinkyo, R. et al. (2011) The Journal of biological chemistry 286, 33021-8. PMID: 21813643 5. Noshiro, M. et al. (1990) FEBS letters 268, 137-40. PMID: 2384150 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
