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| 카탈로그 번호 | HC395012 |
|---|---|
| 설명 |
Recombinant Human DNM1 Protein, N-His (HC395012) expressed in E. coli, spanning Met1-Arg271. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q05193 |
| 단백질 길이 | Met1-Arg271 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 32.02 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DNM1, DNM, Dynamin-1 |
| 배경 | Dynamin-1 (DNM1) is a ~97 kDa protein. Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks. Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis. Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission. May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR. 1. Soulet, F. et al. (2005) Molecular biology of the cell 16, 2058-67. PMID: 15703209 2. Chappie, JS. et al. (2010) Nature 465, 435-40. PMID: 20428113 3. Srinivasan, S. et al. (2018) PLoS biology 16, e2005377. PMID: 29668686 4. van der Bliek, AM. et al. (1993) The Journal of cell biology 122, 553-63. PMID: 8101525 5. Lin, HC. et al. (1996) The Journal of biological chemistry 271, 27979-82. PMID: 8910402 6. Muhlberg, AB. et al. (1997) The EMBO journal 16, 6676-83. PMID: 9362482 7. Kong, L. et al. (2018) Nature 560, 258-262. PMID: 30069048 8. Hinshaw, JE. et al. (1995) Nature 374, 190-2. PMID: 7877694 9. Scaife, R. et al. (1998) Biochemistry 37, 17673-9. PMID: 9922133 10. Bashkirov, PV. et al. (2008) Cell 135, 1276-86. PMID: 19084269 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human DNM1 Protein, N-His
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