中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HD100012 |
|---|---|
| 설명 |
Recombinant Human EEFSEC Protein, N-His (HD100012) expressed in E. coli, spanning Met1-Ala305. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P57772 |
| 단백질 길이 | Met1-Ala305 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 34.63 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | EC:3.6.5.-, EEFSEC, Elongation factor sec, Eukaryotic elongation factor, selenocysteine-tRNA-specific, SELB, Selenocysteine-specific elongation factor |
| 배경 | Selenocysteine-specific elongation factor is a ~65 kDa protein. Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. 1. Dobosz-Bartoszek, M. et al. (2016) Nature communications 7, 12941. PMID: 27708257 2. Hilal, T. et al. (2022) Science (New York, N.Y.) 376, 1338-1343. PMID: 35709277 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
